Author: Rohan Maddamsetti; Daniel T. Johnson; Stephanie J. Spielman; Katherine L. Petrie; Debora S. Marks; Justin R. Meyer
Title: Viral gain-of-function experiments uncover residues under diversifying selection in nature Document date: 2018_1_3
ID: gqhlw20n_16
Snippet: We modified λ strain cI857 (provided by Ing-Nang Wang, SUNY Albany) integrated into the genome of HWEC106 (provided by Harris Wang, Columbia University). We engineered the J substitutions into λ by using MAGE 15,26 on strain HWEC106. MAGE uses the λ-red recombineering system provided on the pKD46 plasmid 27 . For a description of the oligos used, see Supplementary Table S1 . All rights reserved. No reuse allowed without permission......
Document: We modified λ strain cI857 (provided by Ing-Nang Wang, SUNY Albany) integrated into the genome of HWEC106 (provided by Harris Wang, Columbia University). We engineered the J substitutions into λ by using MAGE 15,26 on strain HWEC106. MAGE uses the λ-red recombineering system provided on the pKD46 plasmid 27 . For a description of the oligos used, see Supplementary Table S1 . All rights reserved. No reuse allowed without permission.
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