Author: Yasunori Watanabe; Zachary T. Berndsen; Jayna Raghwani; Gemma E. Seabright; Joel D. Allen; Jason S. McLellan; Ian A. Wilson; Thomas A. Bowden; Andrew B. Ward; Max Crispin
Title: Vulnerabilities in coronavirus glycan shields despite extensive glycosylation Document date: 2020_2_21
ID: bnnt05fn_35
Snippet: Labelled glycans were analysed using a 2.1 mm x 10 mm Acquity BEH Glycan column (Waters) on an Acquity H-Class UPLC instrument (Waters), as performed previously 33 , with fluorescence measurements occurring at λex = 310 nm and λem = 370 nm. Quantification of oligomannose-type glycans was achieved by digestion of fluorescently labelled glycans with Endo H, and clean-up using a PVDF protein-binding membrane (Millipore). Empower 3 software (Waters.....
Document: Labelled glycans were analysed using a 2.1 mm x 10 mm Acquity BEH Glycan column (Waters) on an Acquity H-Class UPLC instrument (Waters), as performed previously 33 , with fluorescence measurements occurring at λex = 310 nm and λem = 370 nm. Quantification of oligomannose-type glycans was achieved by digestion of fluorescently labelled glycans with Endo H, and clean-up using a PVDF protein-binding membrane (Millipore). Empower 3 software (Waters) was used for data processing.
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