Author: Dong, Peng; Ju, Xiangwu; Yan, Yiwu; Zhang, Siya; Cai, Menghua; Wang, Huaishan; Chen, Hui; Hu, Yu; Cui, Lianxian; Zhang, Jianmin; He, Wei
Title: ?d T Cells Provide Protective Function in Highly Pathogenic Avian H5N1 Influenza A Virus Infection Document date: 2018_12_4
ID: 0frb01wq_16
Snippet: Human erythrocytes were separated from whole blood of healthy donors. After isolation and washing, 50 µl of a 0.75% human red blood cell (RBC) suspension was added to 50 µl volumes of 2-fold serial dilutions of purified rHA proteins in a U-bottom 96well plate (BD Falcon, USA; total volume, 100 µl). Agglutination was read after incubation for 60 min at room temperature. As a control, phosphate-buffered saline (PBS) was used instead of rHA......
Document: Human erythrocytes were separated from whole blood of healthy donors. After isolation and washing, 50 µl of a 0.75% human red blood cell (RBC) suspension was added to 50 µl volumes of 2-fold serial dilutions of purified rHA proteins in a U-bottom 96well plate (BD Falcon, USA; total volume, 100 µl). Agglutination was read after incubation for 60 min at room temperature. As a control, phosphate-buffered saline (PBS) was used instead of rHA.
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