?d T Cells Provide Protective Function in Highly Pathogenic Avian H5N1 Influenza A Virus Infection

Dong, Peng; Ju, Xiangwu; Yan, Yiwu; Zhang, Siya; Cai, Menghua; Wang, Huaishan; Chen, Hui; Hu, Yu; Cui, Lianxian; Zhang, Jianmin; He, Wei

The influenza viruses used in this study were an H5N1 [A/Jilin/9/2004 (H5N1)] and an influenza A virus [A/new/Caledonia/20/1999 (H1N1)]. Live virus experiments were performed in biosafety level 3 facilities under governmental and institutional guidelines. Viruses were propagated by inoculation into 10-to 11-day-old specific pathogen-free (SPF) embryonated fowl eggs via the allantoic route. Hemagglutinating allantoic fluid (AF) was collected from eggs and used directly (19) . All animal experiments were conducted with the approval of the Institutional Animal Care and Use Committee of the Institute of Military Veterinary Medicine, Academy of Military Medical Sciences. MDCK cells were purchased from the Peking Union Medical College Cell Culture Center and cultured in DMEM (Gibco Invitrogen, USA) medium supplemented with 10% FBS, 100 U/ml penicillin and 100 µg/ml streptomycin at 37 • C under a 5% carbon dioxide atmosphere. The human lung adenocarcinoma A549 cell line was purchased from the American Type Culture Collection (ATCC) and cultured in F-12/Ham (Gibco Invitrogen, USA) medium supplemented with 10% FBS, 100 U/ml penicillin and 100 µg/ml streptomycin at 37 • C under a 5% carbon dioxide atmosphere.