Author: Dong, Peng; Ju, Xiangwu; Yan, Yiwu; Zhang, Siya; Cai, Menghua; Wang, Huaishan; Chen, Hui; Hu, Yu; Cui, Lianxian; Zhang, Jianmin; He, Wei
Title: ?d T Cells Provide Protective Function in Highly Pathogenic Avian H5N1 Influenza A Virus Infection Document date: 2018_12_4
ID: 0frb01wq_22
Snippet: Measurement of the intracellular Ca 2+ levels was performed in γδ T cells loaded with 2.5 µm fluo-4 AM (Invitrogen, USA) for 45 min at room temperature in HBSS. γδ T cells were washed, resuspended in HBSS and seeded on Lab-Tek TM glass chamber slides (Nunc, Naperville, IL, USA) for 1 h at room temperature. When indicated, HA-his (3 µg/ml), HAF (3 µg/ml) and an anti-CD3 mAb (5 µg/ml) were added (time point: 0 min). Measurements of the intr.....
Document: Measurement of the intracellular Ca 2+ levels was performed in γδ T cells loaded with 2.5 µm fluo-4 AM (Invitrogen, USA) for 45 min at room temperature in HBSS. γδ T cells were washed, resuspended in HBSS and seeded on Lab-Tek TM glass chamber slides (Nunc, Naperville, IL, USA) for 1 h at room temperature. When indicated, HA-his (3 µg/ml), HAF (3 µg/ml) and an anti-CD3 mAb (5 µg/ml) were added (time point: 0 min). Measurements of the intracellular Ca 2+ responses were performed with an UltraVIEW VoX 3D Live Cell Imaging System (PerkinElmer, USA). γδ T cells were illuminated every 20 s and monitored for changes in intracellular Ca 2+ levels by videomicroscopy for 1 h.
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