Author: Dong, Peng; Ju, Xiangwu; Yan, Yiwu; Zhang, Siya; Cai, Menghua; Wang, Huaishan; Chen, Hui; Hu, Yu; Cui, Lianxian; Zhang, Jianmin; He, Wei
Title: ?d T Cells Provide Protective Function in Highly Pathogenic Avian H5N1 Influenza A Virus Infection Document date: 2018_12_4
ID: 0frb01wq_24
Snippet: To determine the surface expression level of sialic acid receptors, γδ T cells were incubated with fluorescein-labeled Sambucus nigra lectin (SNA, Vector, USA; 10 µg/ml; specific for SAα2,6Gal) and fluorescein-labeled Maackia amurensis lectin I (MAL I, Vector, USA; 10 µg/ml; specific for SAα2,3Gal) for 30 min at 4 • C in the dark. γδ T cells were washed, resuspended in PBS and seeded on Lab-Tek TM glass chamber slides (Nunc, Naperville,.....
Document: To determine the surface expression level of sialic acid receptors, γδ T cells were incubated with fluorescein-labeled Sambucus nigra lectin (SNA, Vector, USA; 10 µg/ml; specific for SAα2,6Gal) and fluorescein-labeled Maackia amurensis lectin I (MAL I, Vector, USA; 10 µg/ml; specific for SAα2,3Gal) for 30 min at 4 • C in the dark. γδ T cells were washed, resuspended in PBS and seeded on Lab-Tek TM glass chamber slides (Nunc, Naperville, IL, USA) for 1 h at room temperature. The samples were analyzed using a laser scanning confocal microscope (Olympus, Japan).
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