Author: Dong, Peng; Ju, Xiangwu; Yan, Yiwu; Zhang, Siya; Cai, Menghua; Wang, Huaishan; Chen, Hui; Hu, Yu; Cui, Lianxian; Zhang, Jianmin; He, Wei
Title: ?d T Cells Provide Protective Function in Highly Pathogenic Avian H5N1 Influenza A Virus Infection Document date: 2018_12_4
ID: 0frb01wq_24
Snippet: To determine the surface expression level of sialic acid receptors, γδ T cells were incubated with fluorescein-labeled Sambucus nigra lectin (SNA, Vector, USA; 10 µg/ml; specific for SAα2,6Gal) and fluorescein-labeled Maackia amurensis lectin I (MAL I, Vector, USA; 10 µg/ml; specific for SAα2,3Gal) for 30 min at 4 • C in the dark. γδ T cells were washed, resuspended in PBS and seeded on Lab-Tek TM glass chamber slides (Nunc, Naperville,.....
Document: To determine the surface expression level of sialic acid receptors, γδ T cells were incubated with fluorescein-labeled Sambucus nigra lectin (SNA, Vector, USA; 10 µg/ml; specific for SAα2,6Gal) and fluorescein-labeled Maackia amurensis lectin I (MAL I, Vector, USA; 10 µg/ml; specific for SAα2,3Gal) for 30 min at 4 • C in the dark. γδ T cells were washed, resuspended in PBS and seeded on Lab-Tek TM glass chamber slides (Nunc, Naperville, IL, USA) for 1 h at room temperature. The samples were analyzed using a laser scanning confocal microscope (Olympus, Japan).
Search related documents:
Co phrase search for related documents- µg ml and glass chamber: 1, 2
- µg ml and Lab Tek TM glass chamber: 1
- µg ml and laser scanning: 1, 2, 3, 4, 5, 6
- µg ml and laser scanning confocal microscope: 1, 2
- µg ml and room temperature: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72
- µg ml and surface expression: 1, 2, 3, 4, 5, 6
- confocal microscope and glass chamber: 1, 2, 3
- confocal microscope and laser scanning: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35
- confocal microscope and laser scanning confocal microscope: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35
- confocal microscope and room temperature: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30
- glass chamber and Lab Tek TM glass chamber: 1
- glass chamber and room temperature: 1, 2, 3, 4, 5, 6
- Lab Tek TM glass chamber and room temperature: 1
- laser scanning and room temperature: 1, 2, 3, 4, 5, 6, 7, 8, 9
Co phrase search for related documents, hyperlinks ordered by date