Author: Elsie Yekwa; Chutima Aphibanthammakit; Xavier Carnec; Bruno Coutard; Caroline Picard; Bruno Canard; Sylvain Baize; François Ferron
Title: Arenaviridae exoribonuclease presents genomic RNA edition capacity Document date: 2019_2_8
ID: fvp45ho6_14
Snippet: Supernatants were collected four days post infection. Viral production/titration/infection were repeated iteratively for 10 times. For viral titration, the presence of the viruses was revealed by immunostaining in infected cells with a polyclonal rabbit antibody that recognizes the MOPV Z protein (Agrobio, France) and a phosphatase alkaline-conjugated polyclonal goat anti-rabbit antibody (Sigma) and 1-Step NBT/BCIP substrate (Thermo Fisher scient.....
Document: Supernatants were collected four days post infection. Viral production/titration/infection were repeated iteratively for 10 times. For viral titration, the presence of the viruses was revealed by immunostaining in infected cells with a polyclonal rabbit antibody that recognizes the MOPV Z protein (Agrobio, France) and a phosphatase alkaline-conjugated polyclonal goat anti-rabbit antibody (Sigma) and 1-Step NBT/BCIP substrate (Thermo Fisher scientific, Waltham, MA).
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