Selected article for: "cell culture and protein function"

Author: Wodrich, Harald; Henaff, Daniel; Jammart, Baptist; Segura-Morales, Carolina; Seelmeir, Sigrid; Coux, Olivier; Ruzsics, Zsolt; Wiethoff, Christopher M.; Kremer, Eric J.
Title: A Capsid-Encoded PPxY-Motif Facilitates Adenovirus Entry
  • Document date: 2010_3_19
  • ID: 1mjmttec_48
    Snippet: Mastadenovirus suggests that protein VI fulfils a key function. We suggest that after endocytosis cell or serotype specific capsid disassembly cues regulate exposure, membrane-interaction and ubiquitylation of protein VI (and possibly other capsid proteins). This could facilitate the recruitment of a common cellular microtubule-dependent pathway for retrograde trafficking. This mechanism could be more important in vivo in highly polarized epithel.....
    Document: Mastadenovirus suggests that protein VI fulfils a key function. We suggest that after endocytosis cell or serotype specific capsid disassembly cues regulate exposure, membrane-interaction and ubiquitylation of protein VI (and possibly other capsid proteins). This could facilitate the recruitment of a common cellular microtubule-dependent pathway for retrograde trafficking. This mechanism could be more important in vivo in highly polarized epithelia or neurons where long-range movement is crucial and might be less important in cell culture models [50] . Given the prevalence of viral late domains, ubiquitylation and trafficking towards the MTOC our results may have uncovered a more general mechanism by which viruses and other cargos achieve intracellular delivery and provide a rational to look for further ''early'' functions of PPxY motif containing late-domains in other viral systems.

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