Author: Wodrich, Harald; Henaff, Daniel; Jammart, Baptist; Segura-Morales, Carolina; Seelmeir, Sigrid; Coux, Olivier; Ruzsics, Zsolt; Wiethoff, Christopher M.; Kremer, Eric J.
Title: A Capsid-Encoded PPxY-Motif Facilitates Adenovirus Entry Document date: 2010_3_19
ID: 1mjmttec_31
Snippet: To determine whether any of the ligases specifically interact with protein VI, we used purified cytosol from cells overexpressing GFP-tagged ligases and performed pull-downs with beads coated with recombinant protein VI-wt or VI-M1. Two ligases, Nedd4.1 and Nedd4.2, were highly enriched on VI-wt beads while none of the other ligases showed strong binding to VI-wt-or to VI-M1beads ( Figure 7B ). Taken together, these data suggest a preferential in.....
Document: To determine whether any of the ligases specifically interact with protein VI, we used purified cytosol from cells overexpressing GFP-tagged ligases and performed pull-downs with beads coated with recombinant protein VI-wt or VI-M1. Two ligases, Nedd4.1 and Nedd4.2, were highly enriched on VI-wt beads while none of the other ligases showed strong binding to VI-wt-or to VI-M1beads ( Figure 7B ). Taken together, these data suggest a preferential interaction between Nedd4.1 and Nedd4.2 and the PPxY motif in protein VI, which leads to relocalization of the ligases from the cytoplasm to a membrane compartment.
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