Selected article for: "following day and gfp expression"
Author: Wodrich, Harald; Henaff, Daniel; Jammart, Baptist; Segura-Morales, Carolina; Seelmeir, Sigrid; Coux, Olivier; Ruzsics, Zsolt; Wiethoff, Christopher M.; Kremer, Eric J.
Title: A Capsid-Encoded PPxY-Motif Facilitates Adenovirus Entry
  • Document date: 2010_3_19
    • ID: 1mjmttec_33
    Snippet: To further characterize the interaction between protein VI and the ligases, we knocked down Nedd4.1, Nedd4.2, AIP4/Itch, WWP1 and WWP2 using siRNAs ( Figure 8A ). The cells were then incubated with an Ad5 vector harboring a GFP expression cassette (AdGFP) at a low multiplicity of infection (30 physical particles per cell) for 3 h to achieve approximately 20% transduced cells and limit the time of virus exposure. The following day the percentage o.....
    Document: To further characterize the interaction between protein VI and the ligases, we knocked down Nedd4.1, Nedd4.2, AIP4/Itch, WWP1 and WWP2 using siRNAs ( Figure 8A ). The cells were then incubated with an Ad5 vector harboring a GFP expression cassette (AdGFP) at a low multiplicity of infection (30 physical particles per cell) for 3 h to achieve approximately 20% transduced cells and limit the time of virus exposure. The following day the percentage of GFP-positive cells was quantified by flow cytometry. Most ligase knockdowns had no significant effects on transduction, but Nedd4.2 knockdown diminished transduction by 50% ( Figure 8A ).

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