Author: Wodrich, Harald; Henaff, Daniel; Jammart, Baptist; Segura-Morales, Carolina; Seelmeir, Sigrid; Coux, Olivier; Ruzsics, Zsolt; Wiethoff, Christopher M.; Kremer, Eric J.
Title: A Capsid-Encoded PPxY-Motif Facilitates Adenovirus Entry Document date: 2010_3_19
ID: 1mjmttec_53
Snippet: All sequences for protein VI were derived from Ad serotype 5 (Ad5) and cloned into the Gateway TM compatible entry vector pDONR221. Sequence verified DONR plasmids were used for recombination into Gateway TM compatible destination vectors for N-terminal fusion of mRFP (L30-mRFP, kindly provided by E. Bertrand). Bacterial expression vectors for protein VI are based on pET15b. Site-directed mutagenesis was used to change amino acids 148-PPSY-151 to.....
Document: All sequences for protein VI were derived from Ad serotype 5 (Ad5) and cloned into the Gateway TM compatible entry vector pDONR221. Sequence verified DONR plasmids were used for recombination into Gateway TM compatible destination vectors for N-terminal fusion of mRFP (L30-mRFP, kindly provided by E. Bertrand). Bacterial expression vectors for protein VI are based on pET15b. Site-directed mutagenesis was used to change amino acids 148-PPSY-151 to 148-PGAA-151 in protein VI. N-terminal tagged expression vectors for Nedd4.1, and Nedd4.2 were provided by E. Bertrand [52] and tagged expression vectors for AIP4/Itch and WWP1 were a kind gift of Paul Bieniasz (Rockefeller University, New York). Bacterial expression vectors for catalytically active murine GST-Nedd4.2 and the inactive GST-Nedd4.2-DN was kindly provided by S. Kumar [53] .
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