Selected article for: "flow cytometry and gfp expression"
Author: Wodrich, Harald; Henaff, Daniel; Jammart, Baptist; Segura-Morales, Carolina; Seelmeir, Sigrid; Coux, Olivier; Ruzsics, Zsolt; Wiethoff, Christopher M.; Kremer, Eric J.
Title: A Capsid-Encoded PPxY-Motif Facilitates Adenovirus Entry
  • Document date: 2010_3_19
    • ID: 1mjmttec_56
    Snippet: Depletions were performed in 12-well dishes using 2610 5 U2OS cells. Cells were transfected after 24 and 48 h with 20 pmol of each siRNA duplex per well. Forty-eight hours after the first transfection cells were transduced using 30 physical particles per cell of Ad5-GFP virus for 3 h without prior pre-adsorption. Cells were harvested 24 h later and analyzed by flow cytometry for GFP expression and further processed for western blot analysis to ve.....
    Document: Depletions were performed in 12-well dishes using 2610 5 U2OS cells. Cells were transfected after 24 and 48 h with 20 pmol of each siRNA duplex per well. Forty-eight hours after the first transfection cells were transduced using 30 physical particles per cell of Ad5-GFP virus for 3 h without prior pre-adsorption. Cells were harvested 24 h later and analyzed by flow cytometry for GFP expression and further processed for western blot analysis to verify knock-down efficiency. Acquisitions were done with FACSCali-burH or FACSCantoIIH cytometer (BD Biosciences) and the data were processed and analyzed by the CellQuestH Pro and FACSDIVAH software (BD Biosciences).

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