A Capsid-Encoded PPxY-Motif Facilitates Adenovirus Entry

Wodrich, Harald; Henaff, Daniel; Jammart, Baptist; Segura-Morales, Carolina; Seelmeir, Sigrid; Coux, Olivier; Ruzsics, Zsolt; Wiethoff, Christopher M.; Kremer, Eric J.

To address trafficking using a different approach, we inserted a GFP expression cassette into the FRT site in the E1-deleted region of the wt and the M1 mutant virus (see Figure S3 for details). The GFP expressing viruses showed no difference in the quantitative yield and biochemical composition after large-scale purification. We repeated plaque forming assays ( Figure 2E ) and single round infection assays ( Figure 2F ), this time using GFP expression as the quantification method in non-complementing U2OS cells. We observed a reduction in infectivity in the same order of magnitude as previously (compare Figures 2C with 2E and 2D with 2F). In addition the GFP expression allowed us to follow the plaque formation over time. As shown in Figure S4 the spread of the M1 mutant virus was significantly slower and led to fewer and much smaller plaques ( Figure 2E and Figure S4 ).