A Capsid-Encoded PPxY-Motif Facilitates Adenovirus Entry

Wodrich, Harald; Henaff, Daniel; Jammart, Baptist; Segura-Morales, Carolina; Seelmeir, Sigrid; Coux, Olivier; Ruzsics, Zsolt; Wiethoff, Christopher M.; Kremer, Eric J.

projection. Two concentric circles with 10 mm and 20 mm in diameter were positioned around the MTOC as shown in the left panel. Virus particles were then counted inside the 10 mm radius and in the region between 10-20 mm. The graph to the right shows the relative abundance within the two regions. Distribution for the wild type virus is shown on the left of the graph and for the PPxY mutated virus to the right. The analysis shows that mutated particles are less likely to accumulate at the MTOC then wt particles. The error bar represents cell-to-cell variation (n.15, p,0.05). doi:10.1371/journal.ppat.1000808.g003 Figure 4 . Subcellular localization of protein VI. U2OS cells were transfected with protein VI fused to mRFP, either using wt protein VI (VI-wt, top row), with mutated protein VI (VI-M1, middle row) or with deleted amphipathic helix (VI-DW, bottom row). Cells were co-stained for microtubules. The protein VI-signal is shown in the left column, the microtubule stain is shown in the second column and an overlay of the signals is shown in the third column. Protein VI fusions appear in red, microtubules in green and the nucleus in blue. The right column shows the field of cells from which the insets (small white square) was taken. The scale bar is 10 mm, Please note that the lower panel is a higher magnification. Association of protein VI with microtubules in tubulo-vesicular structures is indicated by arrows in the top row. Further examples of tubulo-vesicular structures can also be seen in Video S1 showing life-cell imaging of VI-wt transfected cells. doi:10.1371/journal.ppat.1000808.g004