Author: Si, Wei; Zhou, Shun; Wang, Zhao; Cui, Shang-jin
Title: A multiplex reverse transcription-nested polymerase chain reaction for detection and differentiation of wild-type and vaccine strains of canine distemper virus Document date: 2010_5_1
ID: 1fw0xiin_13
Snippet: A fragment of 247 and 177 bp was amplified from CDV wild-type strain and the vaccine strain, respectively. Two bands of 247 and 177 bp were detected simultaneously from the mixed genomic RNA of the CDV wild-type and vaccine strains. Amplification was not possible for non-CDV viruses, such as CPV, CAV, CCV, RV, NDV-infected cells, and uninfected cells by RT-nPCR (Fig. 1) ......
Document: A fragment of 247 and 177 bp was amplified from CDV wild-type strain and the vaccine strain, respectively. Two bands of 247 and 177 bp were detected simultaneously from the mixed genomic RNA of the CDV wild-type and vaccine strains. Amplification was not possible for non-CDV viruses, such as CPV, CAV, CCV, RV, NDV-infected cells, and uninfected cells by RT-nPCR (Fig. 1) .
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