Selected article for: "CDV vaccine strain and RT nPCR assay"
Author: Si, Wei; Zhou, Shun; Wang, Zhao; Cui, Shang-jin
Title: A multiplex reverse transcription-nested polymerase chain reaction for detection and differentiation of wild-type and vaccine strains of canine distemper virus
  • Document date: 2010_5_1
    • ID: 1fw0xiin_15
    Snippet: Serial 10-fold dilutions of CDV vaccine strain were subjected to amplification by multiplex RT-nPCR. The lowest limit of detection with this method was shown to be 0.1 TCID 50 . Of the 30 field samples, 20 tested positive for CDV, among which, 15 showed presence of wild-type viruses, and 5 showed presence of vaccine strain. Three independent inter-and intra-assay replicates of the multiplex RT-nPCR gave consistent results, indicating the repeatab.....
    Document: Serial 10-fold dilutions of CDV vaccine strain were subjected to amplification by multiplex RT-nPCR. The lowest limit of detection with this method was shown to be 0.1 TCID 50 . Of the 30 field samples, 20 tested positive for CDV, among which, 15 showed presence of wild-type viruses, and 5 showed presence of vaccine strain. Three independent inter-and intra-assay replicates of the multiplex RT-nPCR gave consistent results, indicating the repeatability of the assay.

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