Selected article for: "detection limit and inter assay"
Author: Si, Wei; Zhou, Shun; Wang, Zhao; Cui, Shang-jin
Title: A multiplex reverse transcription-nested polymerase chain reaction for detection and differentiation of wild-type and vaccine strains of canine distemper virus
  • Document date: 2010_5_1
    • ID: 1fw0xiin_15
    Snippet: Serial 10-fold dilutions of CDV vaccine strain were subjected to amplification by multiplex RT-nPCR. The lowest limit of detection with this method was shown to be 0.1 TCID 50 . Of the 30 field samples, 20 tested positive for CDV, among which, 15 showed presence of wild-type viruses, and 5 showed presence of vaccine strain. Three independent inter-and intra-assay replicates of the multiplex RT-nPCR gave consistent results, indicating the repeatab.....
    Document: Serial 10-fold dilutions of CDV vaccine strain were subjected to amplification by multiplex RT-nPCR. The lowest limit of detection with this method was shown to be 0.1 TCID 50 . Of the 30 field samples, 20 tested positive for CDV, among which, 15 showed presence of wild-type viruses, and 5 showed presence of vaccine strain. Three independent inter-and intra-assay replicates of the multiplex RT-nPCR gave consistent results, indicating the repeatability of the assay.

    Search related documents:
    Co phrase search for related documents
    • CDV vaccine strain and vaccine strain: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10
    • CDV vaccine strain and wild type: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10
    • CDV vaccine strain and wild type virus: 1, 2
    • consistent result and low limit: 1
    • consistent result and method detection: 1, 2
    • consistent result and method detection low limit: 1
    • consistent result and vaccine strain: 1
    • consistent result and wild type: 1, 2
    • consistent result and wild type virus: 1
    • field sample and wild type: 1
    • field sample and wild type virus: 1
    • low limit and method detection: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12
    • low limit and method detection low limit: 1, 2, 3, 4, 5, 6, 7
    • low limit and wild type: 1, 2, 3
    • method detection and vaccine strain: 1, 2, 3, 4, 5, 6
    • method detection and wild type: 1, 2, 3, 4, 5, 6, 7, 8
    • method detection and wild type virus: 1, 2, 3, 4
    • vaccine strain and wild type: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66
    • vaccine strain and wild type virus: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14