Author: Hu, Hao-Teng; Cho, Che-Pei; Lin, Ya-Hui; Chang, Kung-Yao
Title: A general strategy to inhibiting viral -1 frameshifting based on upstream attenuation duplex formation Document date: 2016_1_8
ID: 1u10lpx2_10
Snippet: Synthetic RNAs used in this study were transcribed by T7 RNA polymerase with designed DNA templates using in vitro transcription method (33) . The transcribed RNAs were purified by 20% denaturing polyacrylamide gel electrophoresis in the presence of 8 M urea and the gels of bands containing desired RNA were cut out and electro-eluted using a BIOTRAP device (Schleicher & Schuell). The eluted RNAs were ethanol precipitated and recovered by centrifu.....
Document: Synthetic RNAs used in this study were transcribed by T7 RNA polymerase with designed DNA templates using in vitro transcription method (33) . The transcribed RNAs were purified by 20% denaturing polyacrylamide gel electrophoresis in the presence of 8 M urea and the gels of bands containing desired RNA were cut out and electro-eluted using a BIOTRAP device (Schleicher & Schuell). The eluted RNAs were ethanol precipitated and recovered by centrifugation. Antisense DNA oligonucleotides were chemically synthesized and purchased from Mission Biotech, Taiwan, whereas the 2 OMe-modified RNA oligonucleotides were purchased from TriLink BioTechnologies, Inc., USA. The concentration of all oligonucleotides was determined by UV absorbance.
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