Author: Shehata, Mahmoud M.; Kandeil, Ahmed; Mostafa, Ahmed; Mahmoud, Sara H.; Gomaa, Mokhtar R.; El-Shesheny, Rabeh; Webby, Richard; Kayali, Ghazi; A. Ali, Mohamed
Title: A Recombinant Influenza A/H1N1 Carrying A Short Immunogenic Peptide of MERS-CoV as Bivalent Vaccine in BALB/c Mice Document date: 2019_12_2
ID: 15q6qr4z_30
Snippet: Female (6-8 weeks-old) BALB/c mice were obtained from the animal house at NRC, Egypt. Mice were divided into four groups (seven mice/group). Three groups of mice were intramuscularly immunized with the three forms of inactivated chimeric bivalent 5 + 3, WT 5 + 3, and MERS-CoV vaccines. The remaining group served as a negative control and injected with sterile PBS. All animals received booster doses at 3 wpv. Sera were collected at 0, 2, 4, 6, and.....
Document: Female (6-8 weeks-old) BALB/c mice were obtained from the animal house at NRC, Egypt. Mice were divided into four groups (seven mice/group). Three groups of mice were intramuscularly immunized with the three forms of inactivated chimeric bivalent 5 + 3, WT 5 + 3, and MERS-CoV vaccines. The remaining group served as a negative control and injected with sterile PBS. All animals received booster doses at 3 wpv. Sera were collected at 0, 2, 4, 6, and 8 wpv. The collected sera were tested for antibodies against H1N1pdm09 and MERS-CoV viruses using HAI [31] and PRNT 50 in Vero-E6 cells.
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