Author: Aran Singanayagam; Su-Ling Loo; Maria Calderazzo; Lydia J Finney; Maria-Belen Trujillo Torralbo; Eteri Bakhsoliani; Jason Girkin; Punnam Veerati; Prabuddha S Pathinayake; Kristy S Nichol; Andrew Reid; Joseph Foottit; Sebastian L Johnston; Nathan W Bartlett; Patrick Mallia
Title: Anti-microbial immunity is impaired in COPD patients with frequent exacerbations Document date: 2019_5_9
ID: 72jzqm86_20
Snippet: DNA extraction from total sputum was performed using the FastDNA Spin Kit for Soil (MP Biomedicals, Santa Ana, USA), as per manufacturer instructions. Bead-beating was performed for two cycles of 30 seconds at 6800 rpm (Precellys, Bertin Technologies, Montigny-le-Bretonneux, France). Total 16S bacterial loads were measured using quantitative PCR, as previously described 24 . Viruses were detected as described previously 12 ......
Document: DNA extraction from total sputum was performed using the FastDNA Spin Kit for Soil (MP Biomedicals, Santa Ana, USA), as per manufacturer instructions. Bead-beating was performed for two cycles of 30 seconds at 6800 rpm (Precellys, Bertin Technologies, Montigny-le-Bretonneux, France). Total 16S bacterial loads were measured using quantitative PCR, as previously described 24 . Viruses were detected as described previously 12 .
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