Author: Hu, Hao-Teng; Cho, Che-Pei; Lin, Ya-Hui; Chang, Kung-Yao
Title: A general strategy to inhibiting viral -1 frameshifting based on upstream attenuation duplex formation Document date: 2016_1_8
ID: 1u10lpx2_20
Snippet: Unless otherwise indicated, experiments were performed in triplicate and the relative frameshifting activity was reported as one standard deviation from the mean. Analysis of variance (ANOVA) in each set of data (without or with different amounts of antisenses) was performed. Datasets with an F-value bigger than the critical values from a lookup table for ⣠= 0.05 and P-value smaller than ⣠were then further analyzed by pairwise comparisons t.....
Document: Unless otherwise indicated, experiments were performed in triplicate and the relative frameshifting activity was reported as one standard deviation from the mean. Analysis of variance (ANOVA) in each set of data (without or with different amounts of antisenses) was performed. Datasets with an F-value bigger than the critical values from a lookup table for ⣠= 0.05 and P-value smaller than ⣠were then further analyzed by pairwise comparisons to compute the smallest significant difference (LSD) with a t-test. Additionally, extra experimental data sets were obtained for MERS −1 PRF assay in human 293T cell lysate with or without 10 M of 2 OMe-modified RNA antisense and compared with those of a read-through control (Supplementary Figure S6 ) to perform a statistical analysis suggested for dual-luciferase based assay ( Figure 6B ) (36) .
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