Selected article for: "luciferase assay and lysate cell"

Author: Hu, Hao-Teng; Cho, Che-Pei; Lin, Ya-Hui; Chang, Kung-Yao
Title: A general strategy to inhibiting viral -1 frameshifting based on upstream attenuation duplex formation
  • Document date: 2016_1_8
  • ID: 1u10lpx2_20
    Snippet: Unless otherwise indicated, experiments were performed in triplicate and the relative frameshifting activity was reported as one standard deviation from the mean. Analysis of variance (ANOVA) in each set of data (without or with different amounts of antisenses) was performed. Datasets with an F-value bigger than the critical values from a lookup table for ␣ = 0.05 and P-value smaller than ␣ were then further analyzed by pairwise comparisons t.....
    Document: Unless otherwise indicated, experiments were performed in triplicate and the relative frameshifting activity was reported as one standard deviation from the mean. Analysis of variance (ANOVA) in each set of data (without or with different amounts of antisenses) was performed. Datasets with an F-value bigger than the critical values from a lookup table for ␣ = 0.05 and P-value smaller than ␣ were then further analyzed by pairwise comparisons to compute the smallest significant difference (LSD) with a t-test. Additionally, extra experimental data sets were obtained for MERS −1 PRF assay in human 293T cell lysate with or without 10 M of 2 OMe-modified RNA antisense and compared with those of a read-through control (Supplementary Figure S6 ) to perform a statistical analysis suggested for dual-luciferase based assay ( Figure 6B ) (36) .

    Search related documents:
    Co phrase search for related documents
    • ANOVA variance analysis and data set: 1, 2
    • ANOVA variance analysis and lookup table: 1
    • ANOVA variance analysis and lookup table critical value: 1
    • ANOVA variance analysis and mean standard deviation: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12
    • ANOVA variance and critical value: 1
    • ANOVA variance and data set: 1, 2
    • ANOVA variance and lookup table: 1
    • ANOVA variance and lookup table critical value: 1
    • ANOVA variance and mean standard deviation: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13
    • antisense different amount and different amount: 1
    • cell lysate and dual luciferase: 1, 2
    • cell lysate and frameshifting activity: 1
    • critical value and data set: 1
    • critical value and lookup table: 1
    • critical value and lookup table critical value: 1
    • critical value and mean standard deviation: 1
    • data set and lookup table: 1
    • data set and lookup table critical value: 1
    • data set and mean standard deviation: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12