Selected article for: "PDCoV detection and RT LAMP assay"
Author: Zhang, Fanfan; Ye, Yu; Song, Deping; Guo, Nannan; Peng, Qi; Li, Anqi; Zhou, Xingrong; Chen, Yanjun; Zhang, Min; Huang, Dongyan; Tang, Yuxin
Title: A simple and rapid identification method for newly emerged porcine Deltacoronavirus with loop-mediated isothermal amplification
  • Document date: 2017_9_21
    • ID: 1k9l0z5k_10
    Snippet: In order to ensure the accuracy of the assay, the positive controls used in this study were initially tested for the presence of PDCoV by a conventional RT-PCR established in our laboratory. These results were further confirmed via sequencing and virus isolation methodologies. The results of specificity determination of the RT-LAMP assay ( Fig. 3a , b) demonstrated that only PDCoV as the template could be amplified, and no RT-LAMP amplified produ.....
    Document: In order to ensure the accuracy of the assay, the positive controls used in this study were initially tested for the presence of PDCoV by a conventional RT-PCR established in our laboratory. These results were further confirmed via sequencing and virus isolation methodologies. The results of specificity determination of the RT-LAMP assay ( Fig. 3a , b) demonstrated that only PDCoV as the template could be amplified, and no RT-LAMP amplified products were observed for other reference porcine viral pathogens. Additionally, the RT-LAMP products were confirmed by a digestion analysis with restriction enzyme SmlI (Fig. 3a) , and the results of enzyme digestion analysis were consistent with that from the PDCoV positive control, indicating that the RT-LAMP assay developed was specific for detection of PDCoV.

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