A Capsid-Encoded PPxY-Motif Facilitates Adenovirus Entry

Wodrich, Harald; Henaff, Daniel; Jammart, Baptist; Segura-Morales, Carolina; Seelmeir, Sigrid; Coux, Olivier; Ruzsics, Zsolt; Wiethoff, Christopher M.; Kremer, Eric J.

Video S1 Intracellular dynamics of protein VI. U2OS cells were transfected with VI-wt, fused to mRFP. The movie was acquired using a Nikon TE 2000 microscope equipped with Cascade 512B 2 camera at 1 frame per second. The movie shows rapid intracellular movement of VI-wt depicting compartments resembling vesicular, tubulo-vesicular and reticular structures. Video S2 Comparison of intracellular dynamics for VI-wt vs. VI-M1 vs. VI-wt (+ nocodazole). U2OS cells were transfected with VI-wt (left and right panel) or VI-M1 (middle panel) fused to mRFP. The cell to the right was treated with nocodazole (5 mg/ml) prior to image acquisition. Movies were acquired using a Nikon TE 2000 microscope equipped with Cascade 512B 2 camera at 1 frame per second. The movies were labeled and assembled using imageJ and converted into QuickTime TM movies. All three displayed movies were taking at the same frame rate. The movies show rapid intracellular movement of VI-wt at nearly real-time (left). VI-M1 shows strongly reduced movement (middle). Treatment of VI-wt transfected cells with nocodazole, strongly reduces the VI-wt movement resembling VI-M1 dynamics (right). Found at: doi:10.1371/journal.ppat.1000808.s008 (1.21 MB MOV)