Author: Wodrich, Harald; Henaff, Daniel; Jammart, Baptist; Segura-Morales, Carolina; Seelmeir, Sigrid; Coux, Olivier; Ruzsics, Zsolt; Wiethoff, Christopher M.; Kremer, Eric J.
Title: A Capsid-Encoded PPxY-Motif Facilitates Adenovirus Entry Document date: 2010_3_19
ID: 1mjmttec_60
Snippet: Cells grown on coverslips were rinsed in PBS and fixed with 4% PFA in PBS and blocked/permeabilized with IF-buffer (10% FCS in PBS and 0.1% Saponin). Primary and secondary antibodies where applied to the coverslip in IF-buffer for 1 h each. Cells were mounted in DAKO mounting media containing DAPI and analyzed by confocal-or epifluorescence microscopy. For IF involving microtubule staining cells were treated with crosslinkers prior to fixation. T.....
Document: Cells grown on coverslips were rinsed in PBS and fixed with 4% PFA in PBS and blocked/permeabilized with IF-buffer (10% FCS in PBS and 0.1% Saponin). Primary and secondary antibodies where applied to the coverslip in IF-buffer for 1 h each. Cells were mounted in DAKO mounting media containing DAPI and analyzed by confocal-or epifluorescence microscopy. For IF involving microtubule staining cells were treated with crosslinkers prior to fixation. The following primary antibodies were used in this study: mouse anti-AcTubulin (kind gift from C. Janke, Montpellier), Mouse anti E2A (kind gift of T. Dobner, Hamburg), rabbit anti pericentrin (Abcam) and rabbit anti-protein VI antibodies raised against recombinant protein VI and affinity purified (see supplemental material). Secondary antibodies Alexa546 anti mouse was from Affinity Research and Atto647 anti rabbit was from Sigma.
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