Selected article for: "infectious dose and MOI infection multiplicity"
Author: Shehata, Mahmoud M.; Kandeil, Ahmed; Mostafa, Ahmed; Mahmoud, Sara H.; Gomaa, Mokhtar R.; El-Shesheny, Rabeh; Webby, Richard; Kayali, Ghazi; A. Ali, Mohamed
Title: A Recombinant Influenza A/H1N1 Carrying A Short Immunogenic Peptide of MERS-CoV as Bivalent Vaccine in BALB/c Mice
  • Document date: 2019_12_2
    • ID: 15q6qr4z_26
    Snippet: MDCK-II cells were cultured in 6-well plates overnight to be confluent monolayers. Chimeric bivalent 5 + 3 and WT 5 + 3 viruses were infected with multiplicity of infection (MOI = 0.001) for 1 h at 37 • C in 5% CO 2 incubator. The inoculum was removed and washed with 1× PBS then, a total amount of 2 mL (DMEM infection media) with 1 µg/mL TPCK treated trypsin was added to the cells. The supernatant of infected cells was harvested at specific t.....
    Document: MDCK-II cells were cultured in 6-well plates overnight to be confluent monolayers. Chimeric bivalent 5 + 3 and WT 5 + 3 viruses were infected with multiplicity of infection (MOI = 0.001) for 1 h at 37 • C in 5% CO 2 incubator. The inoculum was removed and washed with 1× PBS then, a total amount of 2 mL (DMEM infection media) with 1 µg/mL TPCK treated trypsin was added to the cells. The supernatant of infected cells was harvested at specific time points and titrated in MDCK-II cells using 50% tissue culture infectious dose (TCID 50 ) according to Reed and Muench protocol [30] .

    Search related documents:
    Co phrase search for related documents
    • Chimeric bivalent and treated trypsin: 1
    • confluent monolayer and infected cell: 1, 2
    • confluent monolayer and plate culture: 1
    • confluent monolayer and tissue culture: 1
    • dmem infection medium and infection medium: 1, 2, 3, 4
    • dmem infection medium and infection multiplicity: 1, 2
    • infected cell and MOI infection multiplicity: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16
    • infected cell and MOI infection multiplicity infect: 1
    • infected cell and PBS wash: 1
    • infected cell and plate culture: 1, 2, 3
    • infected cell and time point: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11
    • infected cell and tissue culture: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26
    • infected cell and tissue culture infectious dose: 1, 2, 3
    • infected cell and treated trypsin: 1, 2, 3
    • infected cell supernatant and MOI infection multiplicity: 1
    • infected cell supernatant and tissue culture: 1, 2
    • infection medium and MOI infection multiplicity: 1, 2, 3
    • infection medium and time point: 1
    • infection medium and tissue culture: 1, 2, 3, 4