Anti-microbial immunity is impaired in COPD patients with frequent exacerbations

Aran Singanayagam; Su-Ling Loo; Maria Calderazzo; Lydia J Finney; Maria-Belen Trujillo Torralbo; Eteri Bakhsoliani; Jason Girkin; Punnam Veerati; Prabuddha S Pathinayake; Kristy S Nichol; Andrew Reid; Joseph Foottit; Sebastian L Johnston; Nathan W Bartlett; Patrick Mallia

The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/632372 doi: bioRxiv preprint FIGURES LEGENDS Figure 1 : COPD patients with frequent exacerbations have reduced anti-viral immunity at clinical stability. A cohort of patients with COPD were monitored prospectively and sputum samples were taken when clinically stable for at least 6 weeks. Patients were stratified according to exacerbation frequency in the preceding year with patients who experienced > 2 exacerbation episodes classified as 'frequent'. Sputum cell mRNA expression of (a) IFNβ, IFNλ2/3 and IFNλ1 was measured by quantitative PCR. (b) Sputum supernatant protein concentrations of CXCL10/IP-10 were measured by ELISA. Sputum cell mRNA expression of interferon-stimulated genes (ISGs) (c) 2'-5'OAS, viperin and Mx1 was measured by quantitative PCR. Box and whisker plots showing median (line within box), IQR (box) and minimum to maximum (whiskers). Statistical comparisons were made using Mann Whitney U test. *p<0.05. ns = non-significant. author/funder. All rights reserved. No reuse allowed without permission.