Author: Si, Wei; Zhou, Shun; Wang, Zhao; Cui, Shang-jin
Title: A multiplex reverse transcription-nested polymerase chain reaction for detection and differentiation of wild-type and vaccine strains of canine distemper virus Document date: 2010_5_1
ID: 1fw0xiin_7
Snippet: RT-nPCR was used to detect the cells infected with CDV vaccine strain, wild-type strain, mixed CDV vaccine and wild-type strains, CPV, CAV, CCV, RV, NDV, and uninfected cells to test its specificity. Extracted RNA from serially diluted (10 4 , 10 3 , 10 2 , 10 1 , 10 0 , 10 -1 , 10 -2 , 10 -3 TCID 50 ) CDV cell cultures (10 6.5 TCID 50 /mL) were assayed by RT-nPCR to determine its sensitivity. RT-nPCR was performed to identify cells infected with.....
Document: RT-nPCR was used to detect the cells infected with CDV vaccine strain, wild-type strain, mixed CDV vaccine and wild-type strains, CPV, CAV, CCV, RV, NDV, and uninfected cells to test its specificity. Extracted RNA from serially diluted (10 4 , 10 3 , 10 2 , 10 1 , 10 0 , 10 -1 , 10 -2 , 10 -3 TCID 50 ) CDV cell cultures (10 6.5 TCID 50 /mL) were assayed by RT-nPCR to determine its sensitivity. RT-nPCR was performed to identify cells infected with CDV vaccine strain, wild-type strain, mixed CDV vaccine and wild-type strains, field samples from Heilongjiang and Jilin province, CPV, CAV, CCV, RV, NDV, and uninfected cells three times to validate the repeatability of the test. 30 samples in Heilongjiang and Jilin provinces, and Fifty-one field samples from dogs, raccoons, foxes, and minks in Shandong provinces were assayed by RT-nPCR; the background of the 51 field samples is listed in table 1. All the positive field samples wild-type strain were confirmed by Rapid test which BioNote, Inc. produced.
Search related documents:
Co phrase search for related documents- cell culture and rapid test: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21
- cell culture and sensitivity determine: 1, 2
- cell culture and specificity test: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12
- cell culture and uninfected cell: 1, 2, 3, 4, 5, 6, 7, 8, 9
- cell culture and vaccine strain: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36
- cell culture and wild type: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74
- cell culture and wild type strain: 1, 2, 3, 4, 5, 6, 7
- field sample and wild type: 1
- Jilin province and Shandong province: 1, 2, 3
- Jilin province and specificity test: 1, 2
- Jilin province and vaccine strain: 1, 2
- Jilin province and wild type: 1
- Jilin province and wild type strain: 1
- mixed CDV vaccine and vaccine strain: 1
- mixed CDV vaccine and wild type: 1
- mixed CDV vaccine and wild type strain: 1
- positive field and rapid test: 1, 2, 3
- positive field and RT nPCR assay: 1
- positive field and specificity test: 1, 2, 3, 4, 5
Co phrase search for related documents, hyperlinks ordered by date