Author: Hu, Hao-Teng; Cho, Che-Pei; Lin, Ya-Hui; Chang, Kung-Yao
Title: A general strategy to inhibiting viral -1 frameshifting based on upstream attenuation duplex formation Document date: 2016_1_8
ID: 1u10lpx2_14
Snippet: Human embryonic kidney HEK-293T cells were cultured as described above. One day before the transfection, 0.5-1 × 10 5 HEK-293T cells per well were plate in a 24-well culture plate with 1000 l growth medium. Transfection was carried out, by adding a mixture of 0.5 g plasmid DNA and jetPEI TM transfection reagent (Polyplus) into each well, according to the manufacturer's instructions. Luciferase activity measurements for transfected 293T cell lysa.....
Document: Human embryonic kidney HEK-293T cells were cultured as described above. One day before the transfection, 0.5-1 × 10 5 HEK-293T cells per well were plate in a 24-well culture plate with 1000 l growth medium. Transfection was carried out, by adding a mixture of 0.5 g plasmid DNA and jetPEI TM transfection reagent (Polyplus) into each well, according to the manufacturer's instructions. Luciferase activity measurements for transfected 293T cell lysates were performed as described below.
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