Alternative conformations of a major antigenic site on RSV F

Jones, Harrison G.; Battles, Michael B.; Lin, Chun-Chi; Bianchi, Siro; Corti, Davide; McLellan, Jason S.

A molecular replacement solution for the 3.50 Ã… complex of DS-Cav1 with RSD5-GL Fab was obtained using PHASER with prefusion-stabilized RSV F variant PR-DM (PDB ID: 5C69) and a chimeric protein Fab model consisting of the heavy and light chains of PDB ID: 1DFB and PDB ID: 1MCO, respectively, separated into the constant and variable domains and without the Fc domain of the 1MCO heavy chain. The structure was built manually in Coot [69] and refined using PHENIX [70] . Rigid-body refinement was initially performed in PHENIX, followed by refining individual B-factors and (x, y, z) coordinates in PHENIX with referencemodel restraints. The reference model was the 2.3 Ã… prefusion F variant PR-DM (PDB ID: 5C69). The structure was built and refined to an R work /R free of 18.3%/20.5% (Table 1) .