Author: Hu, Hao-Teng; Cho, Che-Pei; Lin, Ya-Hui; Chang, Kung-Yao
Title: A general strategy to inhibiting viral -1 frameshifting based on upstream attenuation duplex formation Document date: 2016_1_8
ID: 1u10lpx2_35
Snippet: of −1 PRF efficiency if the drop-off products could not be distinguished from the 0-frame products, the observations from experimental design that moves the 0-frame stop codon away from the location of upstream duplex (in Figure 2C and D) clearly clarified the role of upstream duplexes in −1 PRF attenuation. Furthermore, both a cis-formed upstream hairpin (29) and a trans-formed duplex (this work) can act to attenuate −1 PRF stimulated by .....
Document: of −1 PRF efficiency if the drop-off products could not be distinguished from the 0-frame products, the observations from experimental design that moves the 0-frame stop codon away from the location of upstream duplex (in Figure 2C and D) clearly clarified the role of upstream duplexes in −1 PRF attenuation. Furthermore, both a cis-formed upstream hairpin (29) and a trans-formed duplex (this work) can act to attenuate −1 PRF stimulated by a variety of downstream stimulators in 80S ribosome systems, suggesting both attenuators could share the same −1 PRF attenuation mechanism.
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