Author: Griffiths, Samantha J.; Koegl, Manfred; Boutell, Chris; Zenner, Helen L.; Crump, Colin M.; Pica, Francesca; Gonzalez, Orland; Friedel, Caroline C.; Barry, Gerald; Martin, Kim; Craigon, Marie H.; Chen, Rui; Kaza, Lakshmi N.; Fossum, Even; Fazakerley, John K.; Efstathiou, Stacey; Volpi, Antonio; Zimmer, Ralf; Ghazal, Peter; Haas, Jürgen
Title: A Systematic Analysis of Host Factors Reveals a Med23-Interferon-? Regulatory Axis against Herpes Simplex Virus Type 1 Replication Document date: 2013_8_8
ID: 0lyt8gfq_66
Snippet: A549 cells were transfected in triplicate in black 96-well plates, as described. After incubation at 37uC for 24 h, cells were serumstarved for 24 h by growing in low serum (1%) medium, before cells were left untreated or stimulated with 50 ng/ml IFN-a, IFNb or IFN-c, or 100 ng/ml IFN-l1 or IFN-l2/3 in serum-reduced medium. After 6 h cells were infected with HSV-1 C12 diluted to MOI 0.5 in IFN-containing serum-reduced medium. After 1 h incubation.....
Document: A549 cells were transfected in triplicate in black 96-well plates, as described. After incubation at 37uC for 24 h, cells were serumstarved for 24 h by growing in low serum (1%) medium, before cells were left untreated or stimulated with 50 ng/ml IFN-a, IFNb or IFN-c, or 100 ng/ml IFN-l1 or IFN-l2/3 in serum-reduced medium. After 6 h cells were infected with HSV-1 C12 diluted to MOI 0.5 in IFN-containing serum-reduced medium. After 1 h incubation, virus was removed and media replaced with 100 ml serum-reduced growth medium containing no interferon, IFN-a/b/-c at 50 ng/ml, or IFN-l1/-l2/3 at 100 ng/ml. Replication was monitored as described and normalized to replication in mock-transfected, unstimulated cells.
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