Author: Claus, Claudia; Manssen, Lena; Hübner, Denise; Roßmark, Sarah; Bothe, Viktoria; Petzold, Alice; Große, Claudia; Reins, Mareen; Mankertz, Annette; Frey, Teryl K.; Liebert, Uwe G.
Title: Activation of the Mitochondrial Apoptotic Signaling Platform during Rubella Virus Infection Document date: 2015_11_26
ID: 1rrm4sqa_10
Snippet: In this study, the opening of the mPTP was examined through application of the nonfluorescent, cell-permeant dye calcein-acetoxymethyl (AM), which is converted into the green-fluorescent dye calcein after acetoxymethyl ester hydrolysis by intracellular esterases. Figure 2A shows that overnight incubation of Vero cells with 0.003% H 2 O 2 induces massive loss of calcein from mitochondria due to pore opening. Thereafter a time course experiment was.....
Document: In this study, the opening of the mPTP was examined through application of the nonfluorescent, cell-permeant dye calcein-acetoxymethyl (AM), which is converted into the green-fluorescent dye calcein after acetoxymethyl ester hydrolysis by intracellular esterases. Figure 2A shows that overnight incubation of Vero cells with 0.003% H 2 O 2 induces massive loss of calcein from mitochondria due to pore opening. Thereafter a time course experiment was performed to identify mPTP opening during RV infection. Figure 2B indicates that, with 72 h post-infection as a rather late time point of infection, a notable portion of cells was positive for mPTP opening.
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