An Epithelial Integrin Regulates the Amplitude of Protective Lung Interferon Responses against Multiple Respiratory Pathogens

Meliopoulos, Victoria A.; Van de Velde, Lee-Ann; Van de Velde, Nicholas C.; Karlsson, Erik A.; Neale, Geoff; Vogel, Peter; Guy, Cliff; Sharma, Shalini; Duan, Susu; Surman, Sherri L.; Jones, Bart G.; Johnson, Michael D. L.; Bosio, Catharine; Jolly, Lisa; Jenkins, R. Gisli; Hurwitz, Julia L.; Rosch, Jason W.; Sheppard, Dean; Thomas, Paul G.; Murray, Peter J.; Schultz-Cherry, Stacey

Next we tested whether the β6 KO CD11c + CD11b + AM were a consequence of exposure to an altered lung microenvironment caused by loss of β6, or were caused by a cell-intrinsic for 96 h before obtaining dry weights. Wet weight was normalized to dry weight of each individual lung before calculating fold increase over mice inoculated with PBS (uninfected). Data are pooled from 2 independent experiments with n = 2-3 mice per group. Error bars indicate SEM. p < 0.01 at 5 and 7 dpi by two-way ANOVA with Bonferroni post-test. (D) and (E) At 2, 5, and 7 dpi, mice were sacrificed, BALF collected, total protein levels measured by BCA assay (D) and albumin levels measured by ELISA (E). Data are from 6 independent experiments with n = 2-4 mice per group run in triplicate. Error bars indicate SEM. p < 0.0001 at 7 dpi by two-way ANOVA with Bonferroni post-test. (F) At 8 dpi, mice were i.v. injected with 20 mg/kg Evans blue dye 2 h before sacrifice. Endothelial permeability of the lung was measured by quantification of the amount of dye retained by the tissue. Data was normalized to the dry weight of the lung tissue. Data are pooled from 2 independent experiments with n = 3-4 mice per group run in triplicate. Error bars indicate SEM. p < 0.01 by Student's t test.