A general strategy to inhibiting viral -1 frameshifting based on upstream attenuation duplex formation

Hu, Hao-Teng; Cho, Che-Pei; Lin, Ya-Hui; Chang, Kung-Yao

The stimulation mechanism of −1 PRF was best analyzed in the 70S ribosome system (13, (51) (52) (53) and singlemolecule experiments have linked the existences of downstream −1 PRF stimulator structures to the modulation of several molecular events within a translocation cycle (51) (52) (53) . Ongoing works will test if a trans-formed upstream duplex can replace the functionality of an internal SD·anti-SD interaction in the 70S ribosome for frameshifting regulation aimed for establishing a link between 70S and 80S ribosome in frameshifting regulation via upstream duplexes of different forms. The effects triggered by downstream stimulators during translocation cycle (51-53) could then be examined in the presence of an upstream duplex to help illustrating the mechanisms of both stimulation and attenuation.