Author: Hu, Hao-Teng; Cho, Che-Pei; Lin, Ya-Hui; Chang, Kung-Yao
Title: A general strategy to inhibiting viral -1 frameshifting based on upstream attenuation duplex formation Document date: 2016_1_8
ID: 1u10lpx2_6
Snippet: We used two different −1 PRF reporters to analyze frameshifting efficiency in this study. The p2luc recoding reporter (30) was a gift from Professor John Atkins at the University of Utah, and was used in radioactivity based in vitro translation as well as dual-luciferase based measurement in both in vitro translation and 293T cells for frameshifting efficiency calculation. Additionally, a variant of p2luc was engineered to facilitate radioactiv.....
Document: We used two different −1 PRF reporters to analyze frameshifting efficiency in this study. The p2luc recoding reporter (30) was a gift from Professor John Atkins at the University of Utah, and was used in radioactivity based in vitro translation as well as dual-luciferase based measurement in both in vitro translation and 293T cells for frameshifting efficiency calculation. Additionally, a variant of p2luc was engineered to facilitate radioactivity based −1 PRF activity analysis in vitro. The variant contains a premature −1 frame stop codon 33 nt downstream of the BamHI site of p2luc, and will be translated into a shortened −1 frame product in reticulocyte lysate (31) .
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