Author: Xiao, Haixia; Liu, Li; Zhu, Qingyu; Tan, Zhiwu; Yu, Wenbo; Tang, Xian; Zhan, Dawei; Du, Yanhua; Wang, Haibo; Liu, Di; Li, Zhixin; Yuen, Kwok-Yung; Ho, David D.; Gao, George F.; Chen, Zhiwei
Title: A Replicating Modified Vaccinia Tiantan Strain Expressing an Avian-Derived Influenza H5N1 Hemagglutinin Induce Broadly Neutralizing Antibodies and Cross-Clade Protective Immunity in Mice Document date: 2013_12_17
ID: 0s2gow7a_11
Snippet: Six-week-old female BALB/c mice were used in all animal experiments. Three groups of mice (5 in each group) were immunized with 3.0610 6 plaque formation unit (PFU) of MVTT HA-QH or MVTT HA-AH through intranasal, intramuscular or oral routes at day 0 and day 31, respectively. The same number of mice was included in control groups given either PBS or MVTT S . MVTT S is a recombinant virus expressing the spike glycoprotein of SARS-CoV, as we recent.....
Document: Six-week-old female BALB/c mice were used in all animal experiments. Three groups of mice (5 in each group) were immunized with 3.0610 6 plaque formation unit (PFU) of MVTT HA-QH or MVTT HA-AH through intranasal, intramuscular or oral routes at day 0 and day 31, respectively. The same number of mice was included in control groups given either PBS or MVTT S . MVTT S is a recombinant virus expressing the spike glycoprotein of SARS-CoV, as we recently described [24] . Blood samples from each mouse were collected 2 weeks after each vaccination for measurement of antibody responses. Splenocytes were also collected for measuring cell-mediated immune response after stimulation with an H-2K d -restricted T-cell epitope IYST-VASSL, as previously described by others [27] .
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