Author: Griffiths, Samantha J.; Koegl, Manfred; Boutell, Chris; Zenner, Helen L.; Crump, Colin M.; Pica, Francesca; Gonzalez, Orland; Friedel, Caroline C.; Barry, Gerald; Martin, Kim; Craigon, Marie H.; Chen, Rui; Kaza, Lakshmi N.; Fossum, Even; Fazakerley, John K.; Efstathiou, Stacey; Volpi, Antonio; Zimmer, Ralf; Ghazal, Peter; Haas, Jürgen
Title: A Systematic Analysis of Host Factors Reveals a Med23-Interferon-? Regulatory Axis against Herpes Simplex Virus Type 1 Replication Document date: 2013_8_8
ID: 0lyt8gfq_41
Snippet: The HSV-1 clone collection was cloned by recombinatorial (GATEWAY TM , Invitrogen) and conventional cloning into the bait vector pGBKT7, and screened against a library pooled from 12,381 MGC clones [35] in the pGADT7 prey vector using a semiautomated Y2H assay [77] . Interacting prey cDNAs were identified by sequential blasting of RefSeq, ENSEMBL and Unigene databases. BLAST hits with identical parameters (score, expectation value, length of alig.....
Document: The HSV-1 clone collection was cloned by recombinatorial (GATEWAY TM , Invitrogen) and conventional cloning into the bait vector pGBKT7, and screened against a library pooled from 12,381 MGC clones [35] in the pGADT7 prey vector using a semiautomated Y2H assay [77] . Interacting prey cDNAs were identified by sequential blasting of RefSeq, ENSEMBL and Unigene databases. BLAST hits with identical parameters (score, expectation value, length of alignment) were considered indistinguishable and counted separately. A high-confidence dataset was generated from interaction pairs isolated at least twice, or where the bait interacted with two highly related, non-promiscuous preys.
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