Author: Meliopoulos, Victoria A.; Van de Velde, Lee-Ann; Van de Velde, Nicholas C.; Karlsson, Erik A.; Neale, Geoff; Vogel, Peter; Guy, Cliff; Sharma, Shalini; Duan, Susu; Surman, Sherri L.; Jones, Bart G.; Johnson, Michael D. L.; Bosio, Catharine; Jolly, Lisa; Jenkins, R. Gisli; Hurwitz, Julia L.; Rosch, Jason W.; Sheppard, Dean; Thomas, Paul G.; Murray, Peter J.; Schultz-Cherry, Stacey
Title: An Epithelial Integrin Regulates the Amplitude of Protective Lung Interferon Responses against Multiple Respiratory Pathogens Document date: 2016_8_9
ID: 16e99fuz_55
Snippet: Microarray analysis. RNA was collected from sorted populations using Trizol (Invitrogen), according to the manufacturer's protocol. Total RNA (100 ng) was converted to biotinlabeled cDNA (Ambion WTA protocol) and hybridized to a Mouse Gene 2.0 array (Affymetrix) according to the manufacturer's protocol by the Hartwell Center of St. Jude Children's Research Hospital. Probe signals from scanned arrays were summarized by the RMA method [67] and Gene.....
Document: Microarray analysis. RNA was collected from sorted populations using Trizol (Invitrogen), according to the manufacturer's protocol. Total RNA (100 ng) was converted to biotinlabeled cDNA (Ambion WTA protocol) and hybridized to a Mouse Gene 2.0 array (Affymetrix) according to the manufacturer's protocol by the Hartwell Center of St. Jude Children's Research Hospital. Probe signals from scanned arrays were summarized by the RMA method [67] and Gene Set Enrichment Analysis (GSEA) performed as described [68] using curated pathways from MolSigDB (Broad Institute, Cambridge, MA).
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