Author: Peña, Andrea A; Bols, Niels C; Marshall, Sergio H
Title: An evaluation of potential reference genes for stability of expression in two salmonid cell lines after infection with either Piscirickettsia salmonis or IPNV Document date: 2010_4_14
ID: 0xn6mqh6_16
Snippet: Intracellular pathogens such as bacteria and viruses modulate key cellular processes which may involve changes in reference gene expression [25, 26] . Each pathogen manipulates several cellular transcription pathways in different degrees also according to the affected cell type [25, 26] . Efficient infections with P. salmonis and IPNV have been evidenced by increasing cytopathic effect, as well as by accumulation of bacterial DNA and viral RNA ov.....
Document: Intracellular pathogens such as bacteria and viruses modulate key cellular processes which may involve changes in reference gene expression [25, 26] . Each pathogen manipulates several cellular transcription pathways in different degrees also according to the affected cell type [25, 26] . Efficient infections with P. salmonis and IPNV have been evidenced by increasing cytopathic effect, as well as by accumulation of bacterial DNA and viral RNA over time in both CHSE-214 and RTS11 cell cultures (Figures 1 and 2) . To rule out persistency, we made sure CPE was detectable resulting in productive infection ( Figure 1 ). As well, no primer specific amplification of the ITS region of the bacteria 16S operon and the VP2 fragment of IPNV were evidenced in control samples (Figure 2 ). Despite progressive pathogen replication and accumulation in cultures, the expression of some reference genes remained constant, while others varied.
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