Selected article for: "vaccine wild type strain and wild type strain"
Author: Si, Wei; Zhou, Shun; Wang, Zhao; Cui, Shang-jin
Title: A multiplex reverse transcription-nested polymerase chain reaction for detection and differentiation of wild-type and vaccine strains of canine distemper virus
  • Document date: 2010_5_1
    • ID: 1fw0xiin_3
    Snippet: Primer P4 (5'-ACGTCCTGGAC-CCTAAGTTTTG-3') was used as a shared reverse primer. P5(5'-GGTTTTATAAAAGATT), p6(5'-ATCTA-GAGGTAA-3') were used as the primer specific for different CDV vaccine strains. Primer pairs P1/P4, P2/P4, P3/P4 and P5/P6 were expected to generate a product of 600, 247, 177 bp and 177 bp, respectively. CDV vaccine (CDV-A strain) and wild-type strains are maintained in the Harbin Veterinary Research Institute. A total of 30 field .....
    Document: Primer P4 (5'-ACGTCCTGGAC-CCTAAGTTTTG-3') was used as a shared reverse primer. P5(5'-GGTTTTATAAAAGATT), p6(5'-ATCTA-GAGGTAA-3') were used as the primer specific for different CDV vaccine strains. Primer pairs P1/P4, P2/P4, P3/P4 and P5/P6 were expected to generate a product of 600, 247, 177 bp and 177 bp, respectively. CDV vaccine (CDV-A strain) and wild-type strains are maintained in the Harbin Veterinary Research Institute. A total of 30 field samples (lung, spleen, liver, or bladder) were collected from different farms in Heilongjiang and Jilin provinces of China.

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