Selected article for: "plaque reduction and reduction neutralization test"

Author: Shehata, Mahmoud M.; Kandeil, Ahmed; Mostafa, Ahmed; Mahmoud, Sara H.; Gomaa, Mokhtar R.; El-Shesheny, Rabeh; Webby, Richard; Kayali, Ghazi; A. Ali, Mohamed
Title: A Recombinant Influenza A/H1N1 Carrying A Short Immunogenic Peptide of MERS-CoV as Bivalent Vaccine in BALB/c Mice
  • Document date: 2019_12_2
  • ID: 15q6qr4z_10
    Snippet: Four groups of mice were intramuscularly injected with inactivated chimeric bivalent 5 + 3, WT 5 + 3, MERS-CoV vaccines, and 1× phosphate buffer saline (PBS). The collected sera of vaccinated groups with inactivated chimeric bivalent 5 + 3 and WT 5 + 3 revealed an increase in antibody titers at two weeks post vaccination (wpv). At week 4, the hemagglutination inhibition (HAI) titers waned after booster vaccination at week 3. The chimeric bivalen.....
    Document: Four groups of mice were intramuscularly injected with inactivated chimeric bivalent 5 + 3, WT 5 + 3, MERS-CoV vaccines, and 1× phosphate buffer saline (PBS). The collected sera of vaccinated groups with inactivated chimeric bivalent 5 + 3 and WT 5 + 3 revealed an increase in antibody titers at two weeks post vaccination (wpv). At week 4, the hemagglutination inhibition (HAI) titers waned after booster vaccination at week 3. The chimeric bivalent 5 + 3 vaccine showed a significant increase in mean HAI titer reaching 6.5 and 8.2 log 2 at weeks 6 and 8, respectively, in comparison with control-PBS group. The inactivated WT 5 + 3 control group showed statistically significant increase in HAI titer at weeks 6 and 8 in comparison with control-PBS and MERS-CoV inactivated groups. These results revealed that the vaccinated mice have a potent immunogenic response against rgH1N1 virus as shown in Figure 3 . Plaque reduction neutralization test (PRNT 50 ) showed a high significant neutralizing titer in inactivated MERS-CoV vaccinated mice 1:160 titer (7.3 log 2 ) at 8 wpv. The chimeric bivalent 5 + 3 vaccinated mice showed a significant increase (p value < 0.001) of nAbs against MERS-CoV reaching to 1:160 (7.3 log 2 ) at week 8 compared to the control group, as demonstrated in Figure 4 . The control-PBS and inactivated WT 5 + 3 groups showed no nAbs in their sera against MERS-CoV throughout the duration of experiment.

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