Author: Zhang, Fanfan; Ye, Yu; Song, Deping; Guo, Nannan; Peng, Qi; Li, Anqi; Zhou, Xingrong; Chen, Yanjun; Zhang, Min; Huang, Dongyan; Tang, Yuxin
Title: A simple and rapid identification method for newly emerged porcine Deltacoronavirus with loop-mediated isothermal amplification Document date: 2017_9_21
ID: 1k9l0z5k_12
Snippet: All the samples (N = 192) were examined for the presence of PDCoV by conventional RT-PCR, nested RT-PCR, and RT-LAMP. As indicated in Table 1 , the positive rates of conventional RT-PCR, nested RT-PCR, and RT-LAMP were 32.8% (63/192), 37.5% (72/192), and 38.0% (73/192), respectively. It is indicated that our data are consistent with PDCoV prevalence in Chinese swine [6] . The detailed results of detection (case by case) are listed in Additional f.....
Document: All the samples (N = 192) were examined for the presence of PDCoV by conventional RT-PCR, nested RT-PCR, and RT-LAMP. As indicated in Table 1 , the positive rates of conventional RT-PCR, nested RT-PCR, and RT-LAMP were 32.8% (63/192), 37.5% (72/192), and 38.0% (73/192), respectively. It is indicated that our data are consistent with PDCoV prevalence in Chinese swine [6] . The detailed results of detection (case by case) are listed in Additional file 1: Table S1 . The results demonstrated the RT-LAMP assay established was the most sensitive method among these three assays for detection of PDCoV in field samples.
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