A simple and rapid identification method for newly emerged porcine Deltacoronavirus with loop-mediated isothermal amplification

Zhang, Fanfan; Ye, Yu; Song, Deping; Guo, Nannan; Peng, Qi; Li, Anqi; Zhou, Xingrong; Chen, Yanjun; Zhang, Min; Huang, Dongyan; Tang, Yuxin

PDCoV was first described as a newly emerged coronavirus in swine from rectal swabs in 2012 by Woo [2] . Since then, PDCoV infections have been reported in America, Europe, and Asia, and caused substantial economic losses [4-7, 18, 19] . As a result, it is urgently needed to develop an easy, rapid and highly sensitive diagnostic method for In this study, a RT-LAMP assay was developed and evaluated for PDCoV detection. A set of primers was designed against the conserved coding regions of the N gene of PDCoV and the reaction conditions were optimized. The RT-LAMP assay was able to detect PDCoV with a detection limit of 10 copies, which was 100-fold more sensitive than conventional RT-PCR. Furthermore, the RT-LAMP assay only needs a water bath for 70 min incubation to accomplish efficient amplification, which was much fast and simple when compared with conventional RT-PCR and nested RT-PCR.