Author: Zhang, Fanfan; Ye, Yu; Song, Deping; Guo, Nannan; Peng, Qi; Li, Anqi; Zhou, Xingrong; Chen, Yanjun; Zhang, Min; Huang, Dongyan; Tang, Yuxin
Title: A simple and rapid identification method for newly emerged porcine Deltacoronavirus with loop-mediated isothermal amplification Document date: 2017_9_21
ID: 1k9l0z5k_31
Snippet: To determine the sensitivity of the RT-LAMP assay, the constructed recombinant standards with known concentration were made tenfold serial dilutions (from 1 × 10 8 to 1 × 10 0 copies), and served as the templates for conventional RT-PCR, nested RT-PCR and the RT-LAMP assay. To evaluate the specificity of the RT-LAMP assay, RNAs/DNAs of several important pathogenic viral agents of pigs, including PEDV, TGEV, PKoV, PAsTV, PRRSV, CSFV, and PCV2, w.....
Document: To determine the sensitivity of the RT-LAMP assay, the constructed recombinant standards with known concentration were made tenfold serial dilutions (from 1 × 10 8 to 1 × 10 0 copies), and served as the templates for conventional RT-PCR, nested RT-PCR and the RT-LAMP assay. To evaluate the specificity of the RT-LAMP assay, RNAs/DNAs of several important pathogenic viral agents of pigs, including PEDV, TGEV, PKoV, PAsTV, PRRSV, CSFV, and PCV2, were used in the RT-LAMP assay under optimized reaction conditions. The standards of PDCoV and blank template were served as the positive and negative controls, respectively. All reactions were carried out in triplicates.
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