Selected article for: "detection limit and RT LAMP assay sensitivity"
Author: Zhang, Fanfan; Ye, Yu; Song, Deping; Guo, Nannan; Peng, Qi; Li, Anqi; Zhou, Xingrong; Chen, Yanjun; Zhang, Min; Huang, Dongyan; Tang, Yuxin
Title: A simple and rapid identification method for newly emerged porcine Deltacoronavirus with loop-mediated isothermal amplification
  • Document date: 2017_9_21
    • ID: 1k9l0z5k_8
    Snippet: The kinetic analysis of the turbidity showed that the detection limit of RT-LAMP was 1 × 10 1 copies, which was 100 times higher when compared with conventional RT-PCR (Fig. 2a, c) . As illustrated by Fig. 2b , d, the RT-LAMP assay established in this study presented a similar sensitivity with nested RT-PCR. There were no significant differences in context of the sensitivity between electrophoresis analysis and visual observation of SYBR Green I.....
    Document: The kinetic analysis of the turbidity showed that the detection limit of RT-LAMP was 1 × 10 1 copies, which was 100 times higher when compared with conventional RT-PCR (Fig. 2a, c) . As illustrated by Fig. 2b , d, the RT-LAMP assay established in this study presented a similar sensitivity with nested RT-PCR. There were no significant differences in context of the sensitivity between electrophoresis analysis and visual observation of SYBR Green I staining.

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