Author: Meliopoulos, Victoria A.; Van de Velde, Lee-Ann; Van de Velde, Nicholas C.; Karlsson, Erik A.; Neale, Geoff; Vogel, Peter; Guy, Cliff; Sharma, Shalini; Duan, Susu; Surman, Sherri L.; Jones, Bart G.; Johnson, Michael D. L.; Bosio, Catharine; Jolly, Lisa; Jenkins, R. Gisli; Hurwitz, Julia L.; Rosch, Jason W.; Sheppard, Dean; Thomas, Paul G.; Murray, Peter J.; Schultz-Cherry, Stacey
Title: An Epithelial Integrin Regulates the Amplitude of Protective Lung Interferon Responses against Multiple Respiratory Pathogens Document date: 2016_8_9
ID: 16e99fuz_29
Snippet: A/California/04/2009 (CA/09) H1N1, A/Puerto Rico/8/34 H1N1, A/Anhui/1/2013 H7N9 and A/Hong Kong/483/1991 H5N1 influenza viruses were propagated in 10-day old specific-pathogen-free embroynated chicken eggs and viral titers determined by tissue culture infectious dose 50 (TCID 50 ) on Madin Darby canine kidney cells (MDCK; ATCC CCL-34, Manassas, VA) cells as described [50] and quantitated [60] . Enders strain Sendai virus was propagated as describ.....
Document: A/California/04/2009 (CA/09) H1N1, A/Puerto Rico/8/34 H1N1, A/Anhui/1/2013 H7N9 and A/Hong Kong/483/1991 H5N1 influenza viruses were propagated in 10-day old specific-pathogen-free embroynated chicken eggs and viral titers determined by tissue culture infectious dose 50 (TCID 50 ) on Madin Darby canine kidney cells (MDCK; ATCC CCL-34, Manassas, VA) cells as described [50] and quantitated [60] . Enders strain Sendai virus was propagated as described [61] . D39X S. pneumoniae was grown in C + Y medium to log phase (O.D. 0.4), pelleted, and diluted to the appropriate concentration in phosphate buffered saline pH 7.4 and stored on ice until infection. MDCK cells were cultured in modified Eagle's medium (MEM) (Corning, Manassas, VA) supplemented with 2 mM glutamine (Gibco, Grand Island, NY) and 10% fetal bovine serum (Atlanta Biologicals, Lawrenceville, GA) at 37°C, 5% CO 2 .
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