Selected article for: "pH sodium deoxycholate and sodium deoxycholate"
Author: Meliopoulos, Victoria A.; Van de Velde, Lee-Ann; Van de Velde, Nicholas C.; Karlsson, Erik A.; Neale, Geoff; Vogel, Peter; Guy, Cliff; Sharma, Shalini; Duan, Susu; Surman, Sherri L.; Jones, Bart G.; Johnson, Michael D. L.; Bosio, Catharine; Jolly, Lisa; Jenkins, R. Gisli; Hurwitz, Julia L.; Rosch, Jason W.; Sheppard, Dean; Thomas, Paul G.; Murray, Peter J.; Schultz-Cherry, Stacey
Title: An Epithelial Integrin Regulates the Amplitude of Protective Lung Interferon Responses against Multiple Respiratory Pathogens
  • Document date: 2016_8_9
    • ID: 16e99fuz_43
    Snippet: Immunoblotting Whole lungs were collected at different times post-infection and homogenized in RIPA buffer (50 mM TrisHCl pH 7.5, 150 mM NaCl, 0.1% SDS, 0.5% sodium deoxycholate, 1% TritonX-100) containing protease and phosphatase inhibitors (ThermoScientific, Rockford, IL). After centrifugation at 15000 rpm at 4°C, protein concentration was determined using the Bradford assay (Pierce, Rockford, IL) and equivalent amounts loaded onto 10% Tris-gl.....
    Document: Immunoblotting Whole lungs were collected at different times post-infection and homogenized in RIPA buffer (50 mM TrisHCl pH 7.5, 150 mM NaCl, 0.1% SDS, 0.5% sodium deoxycholate, 1% TritonX-100) containing protease and phosphatase inhibitors (ThermoScientific, Rockford, IL). After centrifugation at 15000 rpm at 4°C, protein concentration was determined using the Bradford assay (Pierce, Rockford, IL) and equivalent amounts loaded onto 10% Tris-glycine SDS-PAGE gels (Lonza, Allendale, NJ). After transfer to nitrocellulose, membranes were probed for integrin β6

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