Author: Peña, Andrea A; Bols, Niels C; Marshall, Sergio H
Title: An evaluation of potential reference genes for stability of expression in two salmonid cell lines after infection with either Piscirickettsia salmonis or IPNV Document date: 2010_4_14
ID: 0xn6mqh6_18
Snippet: For each assay, a standard curve for RTS11 and for CHSE-214 samples was generated by using 10-fold serial dilutions of pooled cDNA, generated from a mix of infected and control samples. Linear correlation coefficient (R 2 ) varying from 0.9910 to 0.9998 and efficiencies between 87% and 96% showed that these assays were suitable for quantitative purposes (see Additional file 1)......
Document: For each assay, a standard curve for RTS11 and for CHSE-214 samples was generated by using 10-fold serial dilutions of pooled cDNA, generated from a mix of infected and control samples. Linear correlation coefficient (R 2 ) varying from 0.9910 to 0.9998 and efficiencies between 87% and 96% showed that these assays were suitable for quantitative purposes (see Additional file 1).
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